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Intrinsically Disordered Protein Analysis pp 57–69Cite as

Visualization of Mobility by Atomic Force Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 896))

Abstract

Intrinsically disordered regions (IDRs) of proteins are very thin and hence hard to be visualized by electron microscopy. Thus far, only high-speed atomic force microscopy (HS-AFM) can visualize them. The molecular movies identify the alignment of IDRs and ordered regions in an intrinsically disordered protein (IDP) and show undulation motion of the IDRs. The visualized tail-like structures contain the information of mechanical properties of the IDRs. Here, we describe methods of HS-AFM visualization of IDPs and methods of analyzing the obtained images to characterize IDRs.

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Acknowledgment

This work was supported by Grant-in-Aid for Basic Research (S) from JSPS, Knowledge Cluster/MEXT—Japan, and Grant-in Aid for Scientific Research on Innovative Areas (Research in a Proposed Research Area)/MEXT—Japan.

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Authors and Affiliations

  1. Department of Physics and Bio-AFM Frontier Research Center, Kanazawa University, Kanazawa, Japan

    Toshio Ando

  2. Bio-AFM Frontier Research Center, Kanazawa University, Kanazawa, Japan

    Noriyuki Kodera

Authors
  1. Toshio Ando
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  2. Noriyuki Kodera
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Corresponding author

Correspondence to Toshio Ando .

Editor information

Editors and Affiliations

  1. DEPT OF MOLECULAR MED, College of Medicine, University of South Florida, BRUCE B DOWNS BLVD 12901, TAMPA, 33612, Florida, USA

    Vladimir N. Uversky

  2. Dept. Biochemistry & Molecular Biology, Center for Computational Biology &, Indiana University, West 10th Street 410, Indianapolis, 46202, Indiana, USA

    A. Keith Dunker

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Ando, T., Kodera, N. (2012). Visualization of Mobility by Atomic Force Microscopy. In: Uversky, V., Dunker, A. (eds) Intrinsically Disordered Protein Analysis. Methods in Molecular Biology, vol 896. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3704-8_4

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  • DOI: https://doi.org/10.1007/978-1-4614-3704-8_4

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-3703-1

  • Online ISBN: 978-1-4614-3704-8

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