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Measurement of HIV Rev-Rev Response Element Functional Activity

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HIV Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2807))

Abstract

Retroviruses must overcome cellular restrictions to the nucleocytoplasmic export of viral mRNAs that retain introns in order to complete their replication cycle. HIV accomplishes this using a system comprised of a trans-acting viral protein, Rev, and a cis-acting RNA secondary structure in the viral genome, the Rev-Response Element (RRE). HIV primary isolates differ with respect to the sequence and functional activity of the Rev-RRE system. Here, we describe a high throughput assay system for analyzing Rev-RRE functional activity using packageable viral vectors.

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Authors and Affiliations

  1. Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia, Charlottesville, VA, USA

    Patrick E. H. Jackson, Godfrey Dzhivhuho, Jing Huang, Marie-Louise Hammarskjold & David Rekosh

  2. Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA

    Patrick E. H. Jackson

  3. Department of Microbiology, Immunology, and Cancer Biology, University of Virginia, Charlottesville, VA, USA

    Godfrey Dzhivhuho, Jing Huang, Marie-Louise Hammarskjold & David Rekosh

Authors
  1. Patrick E. H. Jackson
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  2. Godfrey Dzhivhuho
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  3. Jing Huang
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  4. Marie-Louise Hammarskjold
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  5. David Rekosh
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Corresponding author

Correspondence to Patrick E. H. Jackson .

Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, NY, USA

    Vinayaka R. Prasad

  2. Albert Einstein College of Medicine, Bronx, NY, USA

    Ganjam V. Kalpana

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Jackson, P.E.H., Dzhivhuho, G., Huang, J., Hammarskjold, ML., Rekosh, D. (2024). Measurement of HIV Rev-Rev Response Element Functional Activity. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols . Methods in Molecular Biology, vol 2807. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3862-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3862-0_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3861-3

  • Online ISBN: 978-1-0716-3862-0

  • eBook Packages: Springer Protocols

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