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Functional Analysis of NMDAR Subunit Components in Postsynaptic Currents of Identified Cells and Synapses in Brain Slices

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NMDA Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2799))

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Abstract

Epilepsy is one of the most represented neurological diseases worldwide. However, in many cases, the precise molecular mechanisms of epileptogenesis and ictiogenesis are unknown. Because of their important role in synaptic function and neuronal excitability, NMDA receptors are implicated in various epileptogenic mechanisms. Most of these are subunit specific and require a precise analysis of the subunit composition of the NMDARs implicated. Here, we describe an express electrophysiological method to analyze the contribution of NMDAR subunits to spontaneous postsynaptic activity in identified cells in brain slices using patch clamp whole cell recordings.

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Authors and Affiliations

  1. Mediterranean Institute of Neurobiology (INMED), INSERM, Aix-Marseille University, Marseille, France

    Louison Pineau & Nail Burnashev

Authors
  1. Louison Pineau
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  2. Nail Burnashev
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Correspondence to Nail Burnashev .

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Editors and Affiliations

  1. INSERM, Aix-Marseille University, Mediterranean Institute of Neurobiology (INMED), Marseille, France

    Nail Burnashev

  2. INSERM, Aix-Marseille University, Mediterranean Institute of Neurobiology (INMED), Marseille, France

    Pierre Szepetowski

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Pineau, L., Burnashev, N. (2024). Functional Analysis of NMDAR Subunit Components in Postsynaptic Currents of Identified Cells and Synapses in Brain Slices. In: Burnashev, N., Szepetowski, P. (eds) NMDA Receptors. Methods in Molecular Biology, vol 2799. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3830-9_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3830-9_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3829-3

  • Online ISBN: 978-1-0716-3830-9

  • eBook Packages: Springer Protocols

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