Abstract
Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture methods and could allow the breeding of new varieties with higher production and toleration of adverse environmental effects caused by climate change. Genome editing technology, using CRISPR/Cas9, is a fast and precise method for accelerated plant breeding. It limited its use in indica subspecies because of the recalcitrant response to in vitro culture methods. This chapter describes a protocol for CRISPR/Cas9 editing in indica subspecies, specifically in the CR-5272 variety derived from parental lines IR-822, using Agrobacterium tumefaciens and biolistic transformation.
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References
Gutaker RM, Groen SC, Bellis ES et al (2020) Genomic history and ecology of the geographic spread of rice. Nat Plants 6(5):492–502. https://doi.org/10.1038/s41477-020-0659-6
Tong W, Kim TS, Park YJ (2016) Rice chloroplast genome variation architecture and phylogenetic dissection in diverse Oryza species assessed by whole-genome resequencing. Rice 9(1):1–13. https://doi.org/10.1186/s12284-016-0129-y
Wambugu PW, Brozynska M, Furtado A et al (2015) Relationships of wild and domesticated rices (Oryza AA genome species) based upon whole chloroplast genome sequences. Sci Rep 5:1–9. https://doi.org/10.1038/srep13957
Valdez M, Muñoz M, Vega JR et al (1997) Plant regeneration of Indica Rice (Oryza sativa) cultivars from mature embryo-derived calli. Rev Biol Trop 44–45:13–21
Acknowledgments
This study was financed by the “Espacio de Estudios Avanzados de la Universidad de Costa Rica” (Space for Advanced Studies at the University of Costa Rica) (UCREA; Project No. 801-B7-294) and a fellowship granted to R. Rojas-Vázquez by PPCARN. A. Gatica-Arias acknowledges the Cátedra Humboldt 2023 of the University of Costa Rica for supporting the dissemination of biotechnology for the conservation and sustainable use of biodiversity.
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Rojas-Vásquez, R., Hernández-Soto, A., Arrieta-Espinoza, G., Gatica-Arias, A. (2024). CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272). In: Maghuly, F. (eds) Plant Functional Genomics. Methods in Molecular Biology, vol 2788. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3782-1_15
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DOI: https://doi.org/10.1007/978-1-0716-3782-1_15
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