Abstract
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells, along with protocols for inducing adipogenesis to white or beige adipocytes in this cell population and osteogenic differentiation. Isolation of the adipose stromal vascular fraction cells for flow cytometric analysis is also described.
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Acknowledgments
This work used the Cell Biology and Bioimaging Core facility at Pennington Biomedical Research Center that is supported in part by COBRE (NIH P20 GM135002) and NORC (NIH 5P30DK072476-17) center grants from the National Institutes of Health. Dr. Scott is supported by NIH T32AT004094.
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Scott, M.C. et al. (2024). Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic and Osteogenic Differentiation or Flow Cytometry-Based Analysis. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_6
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DOI: https://doi.org/10.1007/978-1-0716-3762-3_6
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