Abstract
Perivascular cells represent an in vivo counterpart of mesenchymal stromal/stem cells that populate the outer layer of blood vessels. Pericytes in capillaries and microvessels and adventitial cells of large arteries and veins give rise to stem/progenitor cells when isolated and cultured in vitro. These cells have been considered candidate cell types for cell therapy. Adipose tissue, being highly vascularized, dispensable, and easily accessed, is a viable option to obtain perivascular cells for use in research and in clinical trials. Here, we describe our established protocol to extract perivascular cells from human fat through fluorescence-activated cell sorting, which allows for the isolation of defined populations of progenitor cells with high reproducibility.
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We thank the Hao Zhang and JHU flow cytometry facility.
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Thottappillil, N., Gomez-Salazar, M.A., Archer, M., Péault, B., James, A.W. (2024). Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_3
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DOI: https://doi.org/10.1007/978-1-0716-3762-3_3
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