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Repurposing Decellularized Lung to Generate Vascularized Fat

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2783))

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Abstract

Conventional therapies to address critically sized defects in subcutaneous adipose tissue remain a reconstructive challenge for surgeons, largely due to the lack of graft pre-vascularization. Adipose tissue relies on a dense microvasculature network to deliver nutrients, oxygen, nonadipose tissue-derived growth factors, cytokines, and hormones, as well as transporting adipose tissue-derived endocrine signals to other organ systems. This chapter addresses these vascularization issues by combining decellularized lung matrices with a step-wise seeding of patient-specific adipose-derived stem cells and endothelial cells to develop large-volume, perfusable, and pre-vascularized adipose grafts.

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Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA

    Lindsey K. Huff, Zihan Ling, Xi Ren & Rosalyn D. Abbott

  2. Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA, USA

    Megan K. DeBari & Rosalyn D. Abbott

Authors
  1. Lindsey K. Huff
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  2. Zihan Ling
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  3. Megan K. DeBari
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  4. Xi Ren
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  5. Rosalyn D. Abbott
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Corresponding author

Correspondence to Rosalyn D. Abbott .

Editor information

Editors and Affiliations

  1. Obatala Sciences Inc, New Orleans, LA, USA

    Jeffrey Gimble

  2. Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, TX, USA

    Bruce Bunnell

  3. Obatala Sciences Inc, New Orleans, LA, USA

    Trivia Frazier

  4. Obatala Sciences Inc, New Orleans, LA, USA

    Cecilia Sanchez

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Huff, L.K., Ling, Z., DeBari, M.K., Ren, X., Abbott, R.D. (2024). Repurposing Decellularized Lung to Generate Vascularized Fat. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_18

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  • DOI: https://doi.org/10.1007/978-1-0716-3762-3_18

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3761-6

  • Online ISBN: 978-1-0716-3762-3

  • eBook Packages: Springer Protocols

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