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Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2783))

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Abstract

Adipose-derived stromal/stem cells (ASCs) and decellularized adipose tissue (DAT) are adipose tissue products obtained from individuals undergoing fat removal procedures like liposuction, lipectomy, or breast reduction. DAT hydrogel is prepared by removing the cells from the adipose tissue and digesting it to form a liquid material that forms a gel at physiological temperature. ASCs seeded on DAT have displayed osteogenic potential in vitro and in animal models of bone defects. Herein, we describe the methods for preparing DAT hydrogel, ASC seeding in DAT hydrogel, osteogenic differentiation of ASCs, creation of critical-sized femur defect model in mice, its treatment with ASC-DAT hydrogel, and analyses.

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References

  1. Venkataiah VS, Yahata Y, Kitagawa A et al (2021) Clinical applications of cell-scaffold constructs for bone regeneration therapy. Cell 10(10):2687

    Article  CAS  Google Scholar 

  2. Mohiuddin OA, Campbell B, Poche JN et al (2019) Decellularized adipose tissue hydrogel promotes bone regeneration in critical-sized mouse femoral defect model. Front Bioeng Biotechnol 7:211

    Article  PubMed  PubMed Central  Google Scholar 

  3. Clough BH, McCarley MR, Krause U et al (2015) Bone regeneration with osteogenically enhanced mesenchymal stem cells and their extracellular matrix proteins. J Bone Miner Res 30(1):83–94

    Article  CAS  PubMed  Google Scholar 

  4. Schemitsch EH (2017) Size matters: defining critical in bone defect size! J Orthop Trauma 31(Suppl 5):S20–S22. https://doi.org/10.1097/bot.0000000000000978

    Article  PubMed  Google Scholar 

  5. Rahman G, Frazier TP, Gimble JM et al (2022) The emerging use of ASC/scaffold composites for the regeneration of Osteochondral defects. Front Bioeng Biotechnol 10:893992

    Article  PubMed  PubMed Central  Google Scholar 

  6. Harrison MAA, Al-Ghadban SI, O’Donnell BT et al (2022) Chapter 3—establishing the adipose stem cell identity: characterization assays and functional properties. In: Kokai L, Marra K, Rubin JP (eds) Scientific principles of adipose stem cells. Academic Press, pp 23–56

    Chapter  Google Scholar 

  7. Strong AL, Hunter RS, Jones RB et al (2016) Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells. J Transl Med 14(1):27. https://doi.org/10.1186/s12967-016-0776-1

    Article  PubMed  PubMed Central  Google Scholar 

  8. Mohiuddin OA, O’Donnell BT, Poche JN et al (2019) Human adipose-derived hydrogel characterization based on in vitro ASC biocompatibility and differentiation. Stem Cells Int 27(9276398):1

    Article  Google Scholar 

  9. Munmun F, Mohiuddin OA, Hoang VT et al (2022) The role of MEK1/2 and MEK5 in melatonin-mediated actions on osteoblastogenesis, osteoclastogenesis, bone microarchitecture, biomechanics, and bone formation. J Pineal Res 73(2):28

    Article  Google Scholar 

  10. Mohiuddin OA, Motherwell JM, Rogers E et al (2020) Characterization and proteomic analysis of decellularized adipose tissue hydrogels derived from lean and overweight/obese human donors. Adv Biosyst 4(10):11

    Article  Google Scholar 

  11. Roddy E, DeBaun MR, Daoud-Gray A et al (2018) Treatment of critical-sized bone defects: clinical and tissue engineering perspectives. Eur J Orthop Surg Traumatol 28(3):351–362. https://doi.org/10.1007/s00590-017-2063-0

    Article  PubMed  Google Scholar 

  12. Mohiuddin OA, Campbell B, Poche JN et al (2019) Decellularized adipose tissue: biochemical composition, in vivo analysis and potential clinical applications. Adv Exp Med Biol. https://doi.org/10.1007/5584_2019_371

  13. Flynn LE (2010) The use of decellularized adipose tissue to provide an inductive microenvironment for the adipogenic differentiation of human adipose-derived stem cells. Biomaterials 31(17):4715–4724. https://doi.org/10.1016/j.biomaterials.2010.02.046

    Article  CAS  PubMed  Google Scholar 

  14. Clough BH, McCarley MR, Gregory CA (2015) A simple critical-sized femoral defect model in mice. J Vis Exp 97:e52368

    Google Scholar 

  15. Young DA, Ibrahim DO, Hu D et al (2011) Injectable hydrogel scaffold from decellularized human lipoaspirate. Acta Biomater 7(3):1040–1049. https://doi.org/10.1016/j.actbio.2010.09.035

    Article  CAS  PubMed  Google Scholar 

  16. Zhao Y, Fan J, Bai S (2019) Biocompatibility of injectable hydrogel from decellularized human adipose tissue in vitro and in vivo. J Biomed Mater Res B Appl Biomater 107(5):1684–1694. https://doi.org/10.1002/jbm.b.34261

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was conducted at the Tulane University’s School of Medicine (New Orleans, LA, USA) and funded by the Musculoskeletal Transplant Foundation.

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Authors and Affiliations

  1. Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan

    Kainat Ahmed, Haadia Tauseef & Omair A. Mohiuddin

Authors
  1. Kainat Ahmed
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  2. Haadia Tauseef
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  3. Omair A. Mohiuddin
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Corresponding author

Correspondence to Omair A. Mohiuddin .

Editor information

Editors and Affiliations

  1. Obatala Sciences Inc, New Orleans, LA, USA

    Jeffrey Gimble

  2. Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, TX, USA

    Bruce Bunnell

  3. Obatala Sciences Inc, New Orleans, LA, USA

    Trivia Frazier

  4. Obatala Sciences Inc, New Orleans, LA, USA

    Cecilia Sanchez

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Ahmed, K., Tauseef, H., Mohiuddin, O.A. (2024). Combination of Adipose-Derived Stromal/Stem Cells and Decellularized Adipose Tissue Hydrogel for Osteogenic Applications. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3762-3_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3761-6

  • Online ISBN: 978-1-0716-3762-3

  • eBook Packages: Springer Protocols

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