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Analysis of Biomechanical Properties of Adipose-Derived Hydrogels for Adipose-Derived Stromal/Stem Cell-Based Hydrogel Culture

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2783))

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Abstract

With the increase in decellularization of different tissue sources, an understanding of the viscoelastic properties of these soft materials is important for determining practical applications. The purpose of this chapter is to better define a series of experiments to profile important rheological properties for adipose-based hydrogels. While there are numerous mechanical characterizations that are done experimentally, the protocol outlined in this chapter provides a step-wise approach to determine the gelation characteristics and native hydrogel network properties. A more complete understanding of adipose-derived hydrogel mechanical properties would provide vital information for downstream applicability in fields such as disease modeling or soft tissue regeneration.

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Author information

Authors and Affiliations

  1. Department of Biological and Agricultural Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA

    Jorge A. Belgodere

  2. Section of Hematology and Medical Oncology, Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA

    Jorge A. Belgodere

Authors
  1. Jorge A. Belgodere
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Corresponding author

Correspondence to Jorge A. Belgodere .

Editor information

Editors and Affiliations

  1. Obatala Sciences Inc, New Orleans, LA, USA

    Jeffrey Gimble

  2. Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, TX, USA

    Bruce Bunnell

  3. Obatala Sciences Inc, New Orleans, LA, USA

    Trivia Frazier

  4. Obatala Sciences Inc, New Orleans, LA, USA

    Cecilia Sanchez

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Belgodere, J.A. (2024). Analysis of Biomechanical Properties of Adipose-Derived Hydrogels for Adipose-Derived Stromal/Stem Cell-Based Hydrogel Culture. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_12

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  • DOI: https://doi.org/10.1007/978-1-0716-3762-3_12

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3761-6

  • Online ISBN: 978-1-0716-3762-3

  • eBook Packages: Springer Protocols

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