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Isolation of Human Adipose-Derived Stem Cells

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2783))

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Abstract

Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine. We present the protocols from our laboratory for isolating and expanding hASCs. The isolation of hASCs involves the enzymatic digestion of adipose tissue and subsequent culturing of the isolated cells.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Immunology and Genetics, School of Biomedical Sciences, University of North Texas Health Sciences Center, Fort Worth, TX, USA

    Elizabeth Montano, Sara Al-Ghadban & Bruce A. Bunnell

Authors
  1. Elizabeth Montano
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  2. Sara Al-Ghadban
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  3. Bruce A. Bunnell
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Corresponding author

Correspondence to Bruce A. Bunnell .

Editor information

Editors and Affiliations

  1. Obatala Sciences Inc, New Orleans, LA, USA

    Jeffrey Gimble

  2. Department of Microbiology, Immunology and Genetics, University of North Texas Health Science, Fort Worth, TX, USA

    Bruce Bunnell

  3. Obatala Sciences Inc, New Orleans, LA, USA

    Trivia Frazier

  4. Obatala Sciences Inc, New Orleans, LA, USA

    Cecilia Sanchez

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Montano, E., Al-Ghadban, S., Bunnell, B.A. (2024). Isolation of Human Adipose-Derived Stem Cells. In: Gimble, J., Bunnell, B., Frazier, T., Sanchez, C. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 2783. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3762-3_1

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  • DOI: https://doi.org/10.1007/978-1-0716-3762-3_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3761-6

  • Online ISBN: 978-1-0716-3762-3

  • eBook Packages: Springer Protocols

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