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Immunohistochemistry for Cancer Stem Cell Detection: Principles and Methods

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Cancer Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2777))

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Abstract

Cancer stem cells (CSCs) are rare immortal cells within tumors with capabilities of self-renewal, differentiation, and tumorigenicity. CSCs play a pivotal role in the tumor development, progression, relapse, and resistance of anticancer therapy. The technique of choice to detect CSCs in formalin-fixed and paraffin-embedded (FFPE) samples is immunohistochemistry (IHC) since it is inexpensive and widespread in most laboratories. The main aims of this chapter are the description of the protocols and the automated immunohistochemical systems used for the identification of CSCs. Furthermore, a focus on the most common troubleshooting in CSC IHC is provided. Finally, an overview of the main markers of cancer stem cells in several cancer types will be provided.

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Correspondence to Renato Franco .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zannini, G., Franco, R., Zito Marino, F. (2024). Immunohistochemistry for Cancer Stem Cell Detection: Principles and Methods. In: Papaccio, F., Papaccio, G. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 2777. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3730-2_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3730-2_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3729-6

  • Online ISBN: 978-1-0716-3730-2

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