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Isolating Circulating Cancer Stem Cells (CCSCs) from Human Whole Blood

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Cancer Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2777))

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Abstract

Measuring circulating tumor cells (CTCs) or circulating cancer stem cells (CCSCs) in blood, which shed from primary tumors, is a noninvasive method to screen and/or diagnose patients with a high risk of developing metastatic cancers or relapse. Here, we describe an optimized and standardized laboratory method for isolating CCSCs from human blood samples, using cancer-specific stem cell biomarkers (Kantara et al., Lab Invest 95:100–112, 2015). When performing this technique, 0–1 circulating epithelial tumor cells/mL blood should be expected in patients free of malignant adenocarcinomas compared to over 3 circulating cancer stem cells/mL blood in patients positive for malignant adenocarcinomas (Kantara et al., Lab Invest 95:100–112, 2015).

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Acknowledgements

This work was supported by NIH grants CA97959 and CA114264 to Pomila Singh and NASA grants NNX09AM08G and NNJ04HD83G to Robert Ullrich.

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Correspondence to Pomila Singh .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kantara, C., Singh, P. (2024). Isolating Circulating Cancer Stem Cells (CCSCs) from Human Whole Blood. In: Papaccio, F., Papaccio, G. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 2777. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3730-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3730-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3729-6

  • Online ISBN: 978-1-0716-3730-2

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