Abstract
The outer and the inner membranes of the chloroplast envelope, also called OEM and IEM, have distinct lipid and protein compositions. They host molecular systems involved in the biogenesis of the organelle, its cellular function, and its communication with other compartments. Here we describe a method for the isolation of these two membranes starting from intact chloroplast preparations, with two alternative procedures based on the starting material. One was developed from spinach leaves, the other from pea leaves. The two procedures differ in the method used to isolate and rupture chloroplasts and separate each membrane.
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References
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Acknowledgments
This work was supported by the French National Research Agency (GRAL Labex ANR-10-LABEX-04, EUR CBS ANR-17-EURE-0003, Alpalga ANR-20-CE02-0020, DIM ANR-21-CE02-0021).
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Block, M.A., Maréchal, E. (2024). Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms. In: Maréchal, E. (eds) Plastids. Methods in Molecular Biology, vol 2776. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3726-5_8
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DOI: https://doi.org/10.1007/978-1-0716-3726-5_8
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-3726-5
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