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Extraction and Quantification of Lipids from Plant or Algae

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Plastids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2776))

Abstract

In plants and algae, the glycerolipidome changes in response to environmental modifications. For instance, in phosphate starvation, phospholipids are degraded and replaced by non-phosphorus lipids, and in nitrogen starvation, storage lipids accumulate. In addition to the well-known applications of oil crops for food, algae lipids are becoming a model for potential applications in health, biofuel, and green chemistry and are used as a platform for genetic engineering. It is therefore important to measure accurately and quickly the glycerolipid content in plants and algae. Here we describe the methods to extract the lipid and quantify the fatty acid amount of the lipid extract and the different lipid classes that are present in these samples.

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References

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Acknowledgments

This protocol was developed at the LIPANG (Lipid analysis in Grenoble) platform hosted by the LPCV and was supported by the Rhône-Alpes-Auvergne Region, the FEDER fund, and Agence Nationale de la Recherche (ANR-10-LABEX-04 GRAL and ANR-17-EURE-0003 CBH-EUR-GS).

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Correspondence to Juliette Jouhet .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gros, V., Lupette, J., Jouhet, J. (2024). Extraction and Quantification of Lipids from Plant or Algae. In: Maréchal, E. (eds) Plastids. Methods in Molecular Biology, vol 2776. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3726-5_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3726-5_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3725-8

  • Online ISBN: 978-1-0716-3726-5

  • eBook Packages: Springer Protocols

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