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Measuring Replicative Lifespan in Cryptococcus neoformans

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Cryptococcus neoformans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2775))

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Abstract

Advances in understanding cellular aging research have been possible due to the analysis of the replicative lifespan of yeast cells. Studying longevity in the pathogenic yeast Cryptococcus neoformans is essential because old yeast cells with age-related phenotypes accumulate during infection and are associated with increased virulence and antifungal tolerance. Microdissection and microfluidic devices are valuable tools for continuously tracking cells at the single-cell level. In this chapter, we describe the features of these two platforms and outline technical limitations and information to study aging mechanisms while assessing the lifespan of yeast cells.

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Acknowledgments

This work was supported by the National Institutes of Health (NIH 1R01- AI127704-01A1) and the Veterans Administration (VA) Merit (I01BX003741-01A2) to BCF. The contents of this article do not represent the views of the VA or the United States government.

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Authors and Affiliations

  1. Department of Medicine, Division of Infectious Diseases, Stony Brook University, Stony Brook, NY, USA

    Vanessa K. A. Silva & Bettina C. Fries

  2. Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, USA

    Natalia K. Oliveira & Bettina C. Fries

  3. Veterans Administration Medical Center, Northport, NY, USA

    Bettina C. Fries

Authors
  1. Vanessa K. A. Silva
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  2. Natalia K. Oliveira
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  3. Bettina C. Fries
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Corresponding author

Correspondence to Bettina C. Fries .

Editor information

Editors and Affiliations

  1. College of Osteopathic Medicine, Marian University - Indiana, Indianapolis, IN, USA

    Erin E. McClelland

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Silva, V.K.A., Oliveira, N.K., Fries, B.C. (2024). Measuring Replicative Lifespan in Cryptococcus neoformans. In: McClelland, E.E. (eds) Cryptococcus neoformans. Methods in Molecular Biology, vol 2775. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3722-7_25

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  • DOI: https://doi.org/10.1007/978-1-0716-3722-7_25

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3721-0

  • Online ISBN: 978-1-0716-3722-7

  • eBook Packages: Springer Protocols

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