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Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana

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The Plant Endoplasmic Reticulum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2772))

Abstract

In this book chapter, we present a method for microsome isolation from the hypocotyl tissue of dark-grown Arabidopsis thaliana. Microsomes are heterogeneous, vesicle-like membranes, which are, not exclusively, derived but enriched with membranes of the endoplasmic reticulum (ER). Here, we describe the experimental setup, including sample preparation, homogenization, differential centrifugation steps, and quality control measures after microsome isolation.

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References

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Acknowledgments

We are grateful to our team members for their helpful discussions. This work was supported by the German Research Foundation (DFG; 470007283 and CIBSS – EXC-2189 – Project ID 390939984 to J.K.-V.) and the Austrian Science Fund (FWF; P29754 to J.K-V., P33497 to S.W.).

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Correspondence to Seinab Noura .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Noura, S., Kleine-Vehn, J., Waidmann, S. (2024). Organ-Specific Microsomes from Dark-Grown Hypocotyls of Arabidopsis thaliana. In: Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum. Methods in Molecular Biology, vol 2772. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3710-4_29

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  • DOI: https://doi.org/10.1007/978-1-0716-3710-4_29

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3709-8

  • Online ISBN: 978-1-0716-3710-4

  • eBook Packages: Springer Protocols

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