Abstract
The objective of this chapter is to provide an overview of the methods used to investigate the connectivity and structure of the nervous system. These methods allow neuronal cells to be categorized according to their location, shape, and connections to other cells. The Golgi-Cox staining gives a thorough picture of all significant neuronal structures found in the brain that may be distinguished from one another. The most significant characteristic is its three-dimensional integrity since all neuronal structures may be followed continuously from one part to the next. Successions of sections of the brain’s neurons are seen with the Golgi stain. The Golgi method is used to serially segment chosen brain parts, and the resulting neurons are produced from those sections.
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References
Golgi C (1873) Sulla struttura della sostanza grigia del cervello. Gazz Med Ital Lombardia 6:244–246
Koyama Y (2013) The unending fascination with the Golgi method. OA Anat 1:24
Buell SJ (1982) Golgi-Cox and rapid golgi methods as applied to autopsied human brain tissue: widely disparate results. J Neuropathol Exp Neurol 41:500–5007
Angulo A, Fernández E, Merchán JA, Molina M (1996) A reliable method for Golgi staining of retina and brain slices. J Neurosci Methods 66:55–59
Koyama Y, Tohyama M (2012) A modified and highly sensitive Golgi-Cox method to enable complete and stable impregnation of embryonic neurons. J Neurosci Methods 209:58–61
Gibb R, Kolb B (1998) A method for vibratome sectioning of Golgi-Cox-stained whole rat brain. J Neurosci Methods 79:1–4. https://doi.org/10.1016/s0165-0270(97)00163-5
Ranjan A, Mallick BN (2010) A modified method for consistent and reliable Golgi-cox staining in significantly reduced time. Front Neurol 1:157. https://doi.org/10.3389/fneur.2010.00157
Levine ND, Rademacher DJ, Collier TJ, O’Malley JA, Kells AP, San Sebastian W (2013) Advances in thin tissue Golgi-Cox impregnation: fast, reliable methods for multi-assay analyses in rodent and non-human primate brain. J Neurosci Methods 213:214–227
Gull S, Ingrisch I, Tausch S, Witte OW, Schmidt S (2015) Consistent and reproducible staining of glia by a modified Golgi-Cox method. J Neurosci Methods 256:141–150
Zaqout S, Kaindl AM (2016) Golgi-Cox staining step by step. Front Neuroanat 10:38
Garcia-Lopez P, Garcia-Marin V, Freire M (2007) The discovery of dendritic spines by Cajal in 1888 and its relevance in present neuroscience. Prog Neurobiol 83:110–130
Rutledge LT, Duncan J, Beatty N (1969) A study of pyramidal cell axon collaterals in intact and partially isolated adult cerebral cortex. Brain Res 16:15–22
Dubey V, Dey S, Dixit AB, Tripathi M, Chandra PS, Banerjee J (2022) Differential glutamate receptor expression and function in the hippocampus, anterior temporal lobe, and neocortex in a pilocarpine model of temporal lobe epilepsy. Exp Neurol 347:113916
Mehder RH, Bennett BM, Andrew RD (2020) Morphometric analysis of hippocampal and neocortical pyramidal neurons in a mouse model of late-onset Alzheimer’s disease. J Alzheimers Dis 74(4):1069–1083
Ledergerber D, Larkum ME (2010) Properties of layer 6 pyramidal neuron apical dendrites. J Neurosci 30(39):13031–13044
Milatovic D, Montine TJ, Zaja-Milatovic S, Madison JL, Bowman AB, Aschner M (2010) Morphometric analysis in neurodegenerative disorders. Curr Protoc Toxicol Chapter 12:1–12.16
Sholl DA (1953) Dendritic organization in the neurons of the visual and motor cortices of the cat. J Anat 87:387–406
Krstonošić B, Milošević NT, Gudović R (2023) Quantitative analysis of the Golgi impregnated human (neo)striatal neurons: observation of the morphological characteristics followed by an emphasis on the functional diversity of cells. Ann Anat 246:152040
Harris KM, Jensen FE, Tsao B (1992) Three-dimensional structure of dendritic spines and synapses in rat hippocampus (CA1) at postnatal day 15 and adult ages: implications for the maturation of synaptic physiology and long-term potentiation. J Neurosci Off J Soc Neurosci 12(7):2685–2705
Schaefer ML, Perez PJ, Wang M, Gray C, Krall C, Sun X, Hunter E, Skinner J, Johns RA (2020) Neonatal isoflurane anesthesia or disruption of postsynaptic density-95 protein interactions change dendritic spine densities and cognitive function in juvenile mice. Anesthesiology 133(4):812–823
Perez-Cruz C, Nolte MW, Van Gaalen MM, Rustay NR, Termont A, Tanghe A, Kirchhoff F, Ebert U (2011) Reduced spine density in specific regions of CA1 pyramidal neurons in two transgenic mouse models of Alzheimer’s disease. J Neurosci 31:3926–3934
Risher WC, Ustunkaya T, Alvarado JS, Eroglu C (2014) Rapid Golgi analysis method for efficient and unbiased classification of dendritic spines. PLoS One 9:e107591
Acknowledgments
This work is funded by the Department of Biotechnology, Ministry of Science and Technology, Govt. of India [BT/PR20392/MED/122/26/2016], and Indian Council for Medical Research (Grant No. BMI/11(58)/2022).
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Dubey, V., Dixit, A.B., Tripathi, M., Sarat Chandra, P., Banerjee, J. (2024). Quantification of Neuronal Dendritic Spine Density and Lengths of Apical and Basal Dendrites in Temporal Lobe Structures Using Golgi-Cox Staining. In: Ray, S.K. (eds) Neuroprotection. Methods in Molecular Biology, vol 2761. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3662-6_5
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