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Albumen and Yolk Plasma Peptidomics for the Identification and Quantitation of Bioactive Molecules and the Quality Control of Hen Egg Products

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Peptidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2758))

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Abstract

Hen eggs and the corresponding food products are essential components of human diet. In addition to supplying basic nutrients, they contain functional peptides that are released in vivo within the intact raw material following physiological proteolytic events affecting specific proteins or derive from technological processing of albumen and yolk fractions as a result of the dedicated use of proteases from plant and microbial sources. Besides their potential importance for functional applications, peptides released under physiological conditions in intact egg can be used as markers of product storage and deterioration. Therefore, characterization and quantitation of peptides in egg and egg-derived products can be used to implement evaluation of potential bioactivities as well as to assess food product qualitative characteristics. Here, we provide dedicated information on extraction, identification, and quantitative analysis of peptides from albumen and yolk plasma; nano-liquid chromatography-mass spectrometry combined with bioinformatic analysis of resulting raw data by different software tools allowed to assign molecules based on database searching and to evaluate their relative quantity in different samples.

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Acknowledgments

This work was supported by grants from: (1) the Italian National Research Council for the project “Nutrizione, Alimentazione ed Invecchiamento Attivo (NUTRAGE)” (FOE-2021–2022); (2) MUR-PON for the project ARS-01-00783 “Sviluppo di Alimenti Funzionali per l’Innovazione dei Prodotti Alimentari di Tradizione Italiana (ALIFUN)”; (3) the National Recovery and Resilience Plan, mission 4, component 2, investment 1.3, MUR call no. 341/2022 funded by the European Union NextGenerationEU for the project “Research and Innovation Network on Food and Nutrition Sustainability, Safety and Security-Working ON Foods (ON-FOODS),” D.C. 1550/2022, PE00000003; (4) the National Recovery and Resilience Plan, mission 4, component 2, investment 1.3, MUR call no. 341/2022 funded by the European Union NextGenerationEU for the project “One Health Basic and Translational Research Actions Addressing Unmet Needs on Emerging Infectious Diseases (IN-FACT),” D.C. 1554/2022, PE00000007; (5) Agritech National Research Center funded within the European Union NextGenerationEU program (the National Recovery and Resilience Plan, mission 4, component 2, investment 1.4—D.D. 1032/2022, CN00000022). This manuscript reflects only the authors’ views and opinions, neither the European Union nor the European Commission can be considered responsible for them.

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Author notes

  1. Simona Arena and Giovanni Renzone contributed equally to this work.

Authors and Affiliations

  1. Proteomics, Metabolomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, Italy

    Simona Arena, Giovanni Renzone, Valentina Ciaravolo & Andrea Scaloni

Authors
  1. Simona Arena
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  2. Giovanni Renzone
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  3. Valentina Ciaravolo
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  4. Andrea Scaloni
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Corresponding author

Correspondence to Andrea Scaloni .

Editor information

Editors and Affiliations

  1. Department of Bioengineering Sciences, Weihenstephan-Tr. University of Applied Science, Freising, Germany

    Michael Schrader

  2. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA

    Lloyd D. Fricker

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Arena, S., Renzone, G., Ciaravolo, V., Scaloni, A. (2024). Albumen and Yolk Plasma Peptidomics for the Identification and Quantitation of Bioactive Molecules and the Quality Control of Hen Egg Products. In: Schrader, M., Fricker, L.D. (eds) Peptidomics. Methods in Molecular Biology, vol 2758. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3646-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3646-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3645-9

  • Online ISBN: 978-1-0716-3646-6

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