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Detection of Small-Molecule Interactions with Fibrillar Tau Protein Aggregates Using Microscale Thermophoresis

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Tau Protein

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2754))


Aggregated fibrillar tau protein is a pathological hallmark of several neurodegenerative diseases. Small molecules that bind to tau fibrils may be applied for their detection and quantification. This is of great importance as they can potentially be used for earlier diagnosis of disease and disease progression. Microscale thermophoresis (MST) enables the detection of biomolecular interactions in an aqueous environment in which no immobilization of either reaction partner is required. Here, an MST assay methodology is described for the detection of the interaction between a variety of small molecules and tau fibrils. The results of this study demonstrate that MST is a practical methodology to quantify interactions between small molecules and tau fibrillar aggregates.

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The authors thank Prof. Alexander K. Buell for his great help with AFM imaging tau fibrils, Dr. Robert Richardson for his suggestion on preparation tau samples for MST experiments, Katalin Solti for supporting some of the MST experiments, and Prof. Finian Leeper, Mr. Ryan Bentley, and Mr. William Crone for helpful discussions. The authors thank National Institute of Health Research (NIHR, RG64473) Cambridge Biomedical Unit in Dementia, and Hungarian Brain Research Program (2017-1.2.1-NKP-2017-00002), Engineering and Physical Sciences Research Council (EP/R005397/1.) for funding.

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Correspondence to Yanyan Zhao .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zhao, Y., Mikes, A., Tóth, G., Aigbirhio, F. (2024). Detection of Small-Molecule Interactions with Fibrillar Tau Protein Aggregates Using Microscale Thermophoresis. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 2754. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3628-2

  • Online ISBN: 978-1-0716-3629-9

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