Abstract
Geometric morphometrics (GM) enables a quantitative study of shapes and forms allowing the identification and characterization of teratogenic malformations. The GM methodology offers several advantages in comparison to traditional biometric methods, such as higher detail and precision analysis. In this chapter, we describe the recent application of the Procrustes method with ImageJ and MorphoJ programs in the characterization of developmental malformations. With this methodology, we are a step closer to being able to assign molecular pathways or unique signatures to a specific teratogen according to the produced phenotypes or to cluster unknown compounds.
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References
Hallgrímsson BC, Boughner J, Turinsky A et al (2009) Geometric Morphometrics and the study of development. In: Sensen CW, Hallgrímsson B (eds) Advanced imaging in biology and medicine: technology, software environments, applications. Springer, Berlin, pp 319–336
Adams D, Rohlf F, Slice D (2013). A field comes of age: Geometric morphometrics in the 21st century. Hystrix 24(1):7–14. https://doi.org/10.4404/hystrix-24.1-6283
Rutland JW, Bellaire CP, Yao A et al (2021) The expanding role of geometric Morphometrics in craniofacial surgery. J Craniofac Surg 32:1104–1109. https://doi.org/10.1097/SCS.0000000000007362
Ross A (2004) Procrustes analysis. Department of Computer Science and Engineering University of South Carolina, South Carolina
Mitteroecker P, Schaefer K (2022) Thirty years of geometric morphometrics: achievements, challenges, and the ongoing quest for biological meaningfulness. Am J Biol Anthropol 178:181–210. https://doi.org/10.1002/ajpa.24531
Lishchenko F, Jones JB (2021) Application of shape analyses to recording structures of marine organisms for stock discrimination and taxonomic purposes. Front Mar Sci 8:1–26. https://doi.org/10.3389/fmars.2021.667183
Klingenberg CP (2016) Size, shape, and form: concepts of allometry in geometric morphometrics. Dev Genes Evol 226:113–137. https://doi.org/10.1007/s00427-016-0539-2
Slice D (2007) Geometric Morphometrics. Annu Rev Anthropol 36:261–281. https://doi.org/10.1146/annurev.anthro.34.081804.120613
Adams DC, Rohlf FJ, Slice DE (2004) Geometric morphometrics: Ten years of progress following the “revolution.” Ital J Zool 71(1):5–16. https://doi.org/10.1080/11250000409356545
Perez KE, King-Heiden TC (2018) Geometric Morphometrics as a tool to evaluate teratogenic effects in Zebrafish (Danio rerio). In: Félix L (ed) Teratogenicity testing: methods and protocols. Springer, New York, pp 373–391
Claes P, Daniels K, Walters M et al (2012) Dysmorphometrics: the modelling of morphological abnormalities. Theor Biol Med Model 9:1–28. https://doi.org/10.1186/1742-4682-9-5
Latif A, Kuijpers MAR, Rachwalski M et al (2020) Morphological variability in unrepaired bilateral clefts with and without cleft palate evaluated with geometric morphometrics. J Anat 236:425–433. https://doi.org/10.1111/joa.13118
Klingenberg CP (2014) Studying morphological integration and modularity at multiple levels: concepts and analysis. Philos Trans R Soc Lond Ser B Biol Sci 369:1–9. https://doi.org/10.1098/rstb.2013.0249
Harper CM, Goldstein DM, Sylvester AD (2022) Comparing and combining sliding semilandmarks and weighted spherical harmonics for shape analysis. J Anat 240:678–687. https://doi.org/10.1111/joa.13589
Jarque S, Rubio-Brotons M, Ibarra J et al (2020) Morphometric analysis of developing zebrafish embryos allows predicting teratogenicity modes of action in higher vertebrates. Reprod Toxicol 96:337–348. https://doi.org/10.1016/j.reprotox.2020.08.004
Acknowledgments
This work is supported by National Funds from FCT – Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020, and Junior Researcher contract 2021.00458.CEECIND (Luís Félix).
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Lei, M.C., Félix, L., Venâncio, C. (2024). Protocol of Geometric Morphometrics for Teratogenicity Testing. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 2753. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3625-1_28
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DOI: https://doi.org/10.1007/978-1-0716-3625-1_28
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