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A Rapid, Equipment-Free DNA Isolation Method for DNA Barcoding

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DNA Barcoding

Abstract

This rapid, equipment-free DNA isolation procedure using chromatography paper is a simple method that can be performed in less than 30 min and requires no wet lab experience. With minimal expense, it offers an affordable alternative for anyone wanting to explore biodiversity. It also provides an excellent option for use in classrooms or other activities that are time limited. The method works best for plants or lichens, producing stable DNA on Whatman® chromatography paper at room temperature, which can be eluted as needed.

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References

  1. Hebert PD, Cywinska A, Ball SL, deWaard JR (2003) Biological identifications through DNA barcodes. Proc Biol Sci 270(1512):313–321. https://doi.org/10.1098/rspb.2002.2218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Stoeckle M (2003) Taxonomy, DNA, and the barcode of life. Bioscience 53(9):796–797. https://doi.org/10.1641/0006-3568(2003)053[0796:TDATBC]2.0.CO;2

    Article  Google Scholar 

  3. Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, Trau M, Botella JR (2017) Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biol 15(11):e2003916. https://doi.org/10.1371/journal.pbio.2003916

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Marizzi C, Florio A, Lee M, Khalfan M, Ghiban C, Nash B, Dorey J, McKenzie S, Mazza C, Cellini F, Baria C, Bepat R, Cosentino L, Dvorak A, Gacevic A, Guzman-Moumtzis C, Heller F, Holt NA, Horenstein J, Joralemon V, Kaur M, Kaur T, Khan A, Kuppan J, Laverty S, Lock C, Pena M, Petrychyn I, Puthenkalam I, Ram D, Ramos A, Scoca N, Sin R, Gonzalez I, Thakur A, Usmanov H, Han K, Wu A, Zhu T, Micklos DA (2018) DNA barcoding Brooklyn (New York): a first assessment of biodiversity in Marine Park by citizen scientists. PLoS One 13(7):e0199015. https://doi.org/10.1371/journal.pone.0199015

    Article  PubMed  PubMed Central  Google Scholar 

  5. Handelsman J, Elgin S, Estrada M, Hays S, Johnson T, Miller S, Mingo V, Shaffer C, Williams J (2022) Achieving STEM diversity: fix the classrooms. Science 376(6597):1057–1059. https://doi.org/10.1126/science.abn9515

    Article  CAS  PubMed  Google Scholar 

  6. Hyman OJ, Doyle EA, Harsh J, Mott J, Pesce A, Rasoul B, Seifert K, Enke RA (2019) CURE-all: large scale implementation of authentic DNA barcoding research into first-year biology curriculum. CourseSource. https://doi.org/10.24918/cs.2019.10

    Book  Google Scholar 

  7. Hackett J, Pepenella S, Marco CF, Bodt L, Grajales LR, Petracca J, Burke J, Mayle A, Nash B, Micklos DA (this volume) Simple, robust invertebrate DNA barcoding: Chelex-based DNA extraction and optimized COI amplification. In: Desalle R (ed) DNA barcoding, methods and protocols, Methods in molecular biology. Springer, New York

    Google Scholar 

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Correspondence to Bruce Nash .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Pepenella, S. et al. (2024). A Rapid, Equipment-Free DNA Isolation Method for DNA Barcoding. In: DeSalle, R. (eds) DNA Barcoding. Methods in Molecular Biology, vol 2744. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3581-0_31

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  • DOI: https://doi.org/10.1007/978-1-0716-3581-0_31

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3580-3

  • Online ISBN: 978-1-0716-3581-0

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