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High-Throughput Quantitative LC-MS/MS Analysis of Benzodiazepines in Human Urine

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Clinical Applications of Mass Spectrometry in Drug Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2737))

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We hereby present a fast, high-throughput, and clinical LC-MS/MS assay for the simultaneous analysis of benzodiazepines in human urine. The assay is used as both a confirmations and semi-quantitative assay for the abovementioned drugs of abuse following immunoassay urine drug screens. Urine levels are reported in the range of 25 ng/mL–500 ng/mL for each of the 22 analytes, based on a six-level calibration and using a subset (10) of stable isotopically labeled analogues as internal standards. The urine sample is clarified, diluted ten times in internal standard reagent, and thereafter injected into the LC-MS/MS instrument. Reversed-phase liquid chromatography is used to separate the mixture, and the TSQ Endura triple-quadrupole (QqQ) MS instrument performs detection via positive-mode electrospray ionization multiple reaction monitoring.

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Correspondence to Y. Victoria Zhang .

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gqamana, P.P., Zhang, Y.V. (2024). High-Throughput Quantitative LC-MS/MS Analysis of Benzodiazepines in Human Urine. In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 2737. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3540-7

  • Online ISBN: 978-1-0716-3541-4

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