Abstract
Phage-host interactions are commonly evaluated by culture-based methods. However, these techniques are very laborious and time-consuming. Therefore, other time-efficient, not labor-intensive, and cost-effective methods have been developed.
This chapter describes the methodology used to assess the susceptibility of planktonic cultures of bacteria to phage infection and to study their interactions over time by flow cytometry.
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Acknowledgments
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and Project PTDC/BIA-MIC/2312/2020. Luís D. R. Melo acknowledges funding from the FCT through the Scientific Employment Stimulus Program (2021.00221.CEECIND).
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Silva, M.D., Melo, L.D.R. (2024). Phage-Host Interaction Analysis Using Flow Cytometry. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 2734. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3523-0_8
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DOI: https://doi.org/10.1007/978-1-0716-3523-0_8
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