Abstract
In-vivo cross-linking of biotinylated peptides is a technique to analyze the interaction of small proteins or peptide ligands with their corresponding receptors. Here, we describe an in-vivo method in which leaves of living plants, transiently expressing receptor proteins, are infiltrated with biotinylated peptides. The interaction between ligand and receptor is irreversibly fixed by the infiltration of a cross-linking agent. Subsequently, co-immunoprecipitation is used to pull down the receptor-ligand pair. After western blotting, the biotin tag of the ligand peptide cross-linked to the receptor can be detected by streptavidin-AP conjugate on the membrane.
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Burggraf, R., Albert, M. (2024). In-vivo Cross-linking of Biotinylated Peptide Ligands to Cell Surface Receptors. In: Schaller, A. (eds) Plant Peptide Hormones and Growth Factors. Methods in Molecular Biology, vol 2731. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3511-7_16
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DOI: https://doi.org/10.1007/978-1-0716-3511-7_16
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