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Primary Human Nasal Epithelial Cell Culture

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Cilia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2725))

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Abstract

Primary human nasal epithelial cell (HNEC) cultures serve as an excellent model in studying physiology, disease, and therapy related to the respiratory tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.

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Author notes

  1. Authors Mystica Terrance and Tarini Gunawardena have equally contributed to this chapter.

Authors and Affiliations

  1. The Hospital for Sick Children, Toronto, ON, Canada

    Mystica Terrance, Tarini Gunawardena, Hong Ouyang, Julie Avolio, Wenming Duan, Sowmya Thanikachalam & Theo J. Moraes

Authors
  1. Mystica Terrance
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  2. Tarini Gunawardena
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  3. Hong Ouyang
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  4. Julie Avolio
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  5. Wenming Duan
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  6. Sowmya Thanikachalam
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  7. Theo J. Moraes
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Corresponding author

Correspondence to Theo J. Moraes .

Editor information

Editors and Affiliations

  1. Medical Research Council Toxicology Unit, School of Biological Sciences, University of Cambridge, Cambridge, UK

    Vito Mennella

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Terrance, M. et al. (2024). Primary Human Nasal Epithelial Cell Culture. In: Mennella, V. (eds) Cilia. Methods in Molecular Biology, vol 2725. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3507-0_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3507-0_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3506-3

  • Online ISBN: 978-1-0716-3507-0

  • eBook Packages: Springer Protocols

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