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Imaging the Tumor Antioxidant Response with [18F]FSPG PET

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Positron Emission Tomography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2729))

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Abstract

(4S)-4-(3-[18F]Fluoropropyl)-L-glutamic acid ([18F]FSPG) is a flourine-18 labeled glutamate analog that enables the noninvasive in vivo imaging of cellular redox status. [18F]FSPG is transported across the cell membrane by the cystine/glutamate antiporter, system xc−, whose expression is upregulated in multiple cancer types. The requirement of cystine for the biosynthesis of glutathione, a major antioxidant, connects [18F]FSPG tissue retention to the intracellular redox response via system xc− activity. We herein describe the use of [18F]FSPG positron emission tomography (PET) to image the tumor antioxidant response and highlight key methodological considerations.

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Authors and Affiliations

  1. School of Biomedical Engineering & Imaging Sciences, King’s College London, London, UK

    Oskar Vilhelmsson Timmermand & Timothy H. Witney

Authors
  1. Oskar Vilhelmsson Timmermand
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  2. Timothy H. Witney
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Correspondence to Timothy H. Witney .

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Editors and Affiliations

  1. School of Biomedical Engineering and Imaging Sciences, King’s College London, London, UK

    Timothy H. Witney

  2. Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada

    Adam J. Shuhendler

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Timmermand, O.V., Witney, T.H. (2024). Imaging the Tumor Antioxidant Response with [18F]FSPG PET. In: Witney, T.H., Shuhendler, A.J. (eds) Positron Emission Tomography. Methods in Molecular Biology, vol 2729. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3499-8_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3499-8_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3498-1

  • Online ISBN: 978-1-0716-3499-8

  • eBook Packages: Springer Protocols

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