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Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery

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Therapeutic Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2720))

Abstract

Nature provides an abundance of proteins whose structures and reactivity have been perfected through evolution to perform specific tasks necessary for biological function. The structural and functional properties of many natural proteins are quite valuable for the construction and customization of drug delivery vehicles. Self-assembling protein nanoparticle platforms are particularly useful scaffolds, as their multi-subunit designs allow the attachment of a high density of modifying molecules such as cell-binding ligands that provide avidity for targeting and facilitate encapsulation of large quantities of therapeutic payload. We explored SpyCatcher/SpyTag conjugation as a system to modify hepatitis B virus (HBV)-like particles (HBV VLPs). Using this simple decoration strategy, we demonstrated efficient and cell-selective killing of inflammatory breast cancer cells via delivery of yeast cytosine deaminase suicide enzymes combined with 5-fluoro-cytosine prodrugs.

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Acknowledgments

This review was supported by grants from NSF (CBE1911950 and DMR1609621).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA

    Millicent O. Sullivan & Wilfred Chen

Authors
  1. Millicent O. Sullivan
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  2. Wilfred Chen
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Corresponding authors

Correspondence to Millicent O. Sullivan or Wilfred Chen .

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Editors and Affiliations

  1. Department of Chemical & Biomolecular Engineering, University of Delaware, Newark, DE, USA

    Millicent O. Sullivan

  2. Department of Molecular Genetics and Microbiology School of Medicine, University of New Mexico, Albuquerque, NM, USA

    Bryce Chackerian

  3. Department of Chemical & Biomolecular Engineering, University of Delaware, Newark, DE, USA

    Wilfred Chen

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sullivan, M.O., Chen, W. (2024). Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery. In: Sullivan, M.O., Chackerian, B., Chen, W. (eds) Therapeutic Proteins. Methods in Molecular Biology, vol 2720. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3469-1_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3469-1_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3468-4

  • Online ISBN: 978-1-0716-3469-1

  • eBook Packages: Springer Protocols

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