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PAR-CLIP Assay in Ferroptosis

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Ferroptosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2712))

Abstract

Ferroptosis is a regulatory cell death process that is accompanied by large amounts of iron ion accumulation and lipid peroxidation. Photoactivated ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) is a method used to identify the binding sites of RNA-binding proteins (RBPs) on target RNAs with high resolution at the nucleotide level. By inserting photosensitive ribonucleoside analogs into new RNA transcripts of living cells, characteristic mutations can be generated during reverse transcription and be used to accurately locate the crosslinking position of RNAs and RBPs. The use of PAR-CLIP to detect interactions and determine precise crosslinking sites between RNAs and RBPs, or to search for RNAs upstream or downstream of ferroptosis pathways genes through known proteins, can help to clarify and verify the occurrence and regulation mechanisms of the various signaling pathways of ferroptosis. Furthermore, it may reveal new targets for ferroptosis detection and improve the treatment efficiency of ferroptosis-related diseases such as cancer and neurodegenerative diseases. Here, we introduce a specific PAR-CLIP protocol for monitoring the ferroptosis process.

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Author information

Author notes

  1. Authors Xiangfei Xue, Manyuan Wang, Xiao Zhang have equally contributed to this chapter.

Authors and Affiliations

  1. Shanghai Institute of Thoracic Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Xiangfei Xue, Xiao Zhang, Lifang Ma & Jiayi Wang

  2. College of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Manyuan Wang

  3. Department of Clinical Laboratory, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Lifang Ma & Jiayi Wang

Authors
  1. Xiangfei Xue
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  2. Manyuan Wang
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  3. Xiao Zhang
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  4. Lifang Ma
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  5. Jiayi Wang
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Corresponding author

Correspondence to Jiayi Wang .

Editor information

Editors and Affiliations

  1. Centre de Recherche des Cordeliers, Université de Paris, Paris, France

    Guido Kroemer

  2. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Daolin Tang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Xue, X., Wang, M., Zhang, X., Ma, L., Wang, J. (2023). PAR-CLIP Assay in Ferroptosis. In: Kroemer, G., Tang, D. (eds) Ferroptosis. Methods in Molecular Biology, vol 2712. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3433-2_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3433-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3432-5

  • Online ISBN: 978-1-0716-3433-2

  • eBook Packages: Springer Protocols

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