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Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP

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Ferroptosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2712))

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Abstract

Ferroptosis is emerging as a promising strategy for suppressing multiple types of human cancers. Rapid and accurate assessment of the relative sensitivity to ferroptosis in biological samples will accelerate the development of ferroptosis-targeted therapies. We previously demonstrated that photochemical activation of membrane lipid peroxidation (PALP) that uses high-power lasers to induce localized polyunsaturated fatty acyl (PUFA)-lipid peroxidation can efficiently report ferroptosis sensitivity in live cells and tissues in situ. Here, we describe the experimental details for PALP analysis, including preparation of tissue sections, preparation of fluorescent lipid peroxidation reporter, sample staining, lipid peroxidation induced by laser source, and data processing. We envision predicting the relative sensitivity to ferroptosis of cellular and tissue samples is potentially useful for basic research and clinical investigations.

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Declaration of Interests

Y.Z. is a consultant of Keen Therapeutics. The remaining authors declare no competing interests. A patent describing the utility of PALP in tissue samples was filed by Y.Z., F.W., N.N., et al.

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Correspondence to Yilong Zou .

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Wang, F., Naowarojna, N., Zou, Y. (2023). Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP. In: Kroemer, G., Tang, D. (eds) Ferroptosis. Methods in Molecular Biology, vol 2712. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3433-2_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3433-2_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3432-5

  • Online ISBN: 978-1-0716-3433-2

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