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Flow Cytometric Analysis of Regulated Cell Death

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Ferroptosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2712))

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Abstract

Cell death is a crucial and fundamental process in the biology of all living organisms and plays an essential role in developmental, cellular, and molecular biology. With a deeper understanding of the mechanisms of different types of cell death, quantitative experiments are becoming increasingly necessary to analyze the dynamic changes and coordinate physiological processes. Flow cytometry is the most widely used method for detecting and quantifying cell processes in mammalian cells, providing a comprehensive and high-throughput approach, even at the single-cell level. This chapter provides a brief overview of guidelines for performing flow cytometry in the detection of regulated cell deaths, including apoptosis, autophagy, ferroptosis, pyroptosis, and immunogenic cell death.

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Author information

Authors and Affiliations

  1. Department of Trauma Medical Center, State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, Army Medical University, Chongqing, China

    Siyuan Huang & Ling Zeng

Authors
  1. Siyuan Huang
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  2. Ling Zeng
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Corresponding author

Correspondence to Ling Zeng .

Editor information

Editors and Affiliations

  1. Centre de Recherche des Cordeliers, Université de Paris, Paris, France

    Guido Kroemer

  2. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Daolin Tang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Huang, S., Zeng, L. (2023). Flow Cytometric Analysis of Regulated Cell Death. In: Kroemer, G., Tang, D. (eds) Ferroptosis. Methods in Molecular Biology, vol 2712. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3433-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3433-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3432-5

  • Online ISBN: 978-1-0716-3433-2

  • eBook Packages: Springer Protocols

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