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Quality Control of Chemogenomic Library Using LC-MS

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Chemogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2706))

Abstract

In chemical biology, using compounds with incorrect identity or insufficient purity can lead to misleading biological activity data. Chemical quality control for confirmation of purity and compound identity is thus central to chemogenomics. We have established a medium-throughput LC-MS-based semi-automated quality control (QC) workflow with a minimal requirement for materials suitable for chemogenomics and other small molecule libraries. This rapid method can cover a broad chemical space of small organic compounds with diverse physicochemical properties such as polarity or lipophilicity.

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References

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Author information

Authors and Affiliations

  1. Institute for Pharmaceutical Chemistry, Structural Genomics Consortium, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany

    Václav Němec & Stefan Knapp

Authors
  1. Václav Němec
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  2. Stefan Knapp
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Corresponding author

Correspondence to Stefan Knapp .

Editor information

Editors and Affiliations

  1. Department of Pharmacy, Ludwig-Maximilians-Universität München, Munich, Germany

    Daniel Merk

  2. Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany

    Apirat Chaikuad

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Němec, V., Knapp, S. (2023). Quality Control of Chemogenomic Library Using LC-MS. In: Merk, D., Chaikuad, A. (eds) Chemogenomics. Methods in Molecular Biology, vol 2706. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3397-7_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3397-7_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3396-0

  • Online ISBN: 978-1-0716-3397-7

  • eBook Packages: Springer Protocols

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