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Long-Range Polymerase Chain Reaction

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2967))


Polymerase chain reaction (PCR) is a laboratory technique used to amplify a targeted region of DNA, demarcated by a set of oligonucleotide primers. Long-range PCR is a form of PCR optimized to facilitate the amplification of large fragments. Using the adapted long-range PCR protocol described in this chapter, we were able to generate PCR products of 6.6, 7.2, 13, and 20 kb from human genomic DNA samples. For some of the long PCRs, successful amplification was not possible without the use of PCR enhancers. Thus, we also evaluated the impact of some enhancers on long-range PCR and included the findings as part of this updated chapter.

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Correspondence to Eng Wee Chua .

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kee, P.S., Karunanathie, H., Maggo, S.D.S., Kennedy, M.A., Chua, E.W. (2023). Long-Range Polymerase Chain Reaction. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 2967. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3357-1

  • Online ISBN: 978-1-0716-3358-8

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