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An In Vitro Model for Assessing Acute Lung Injury During Pancreatitis Development Using Primary Mouse Cell Co-cultures

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Inflammation and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2691))

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Abstract

Acute pancreatitis is a serious inflammatory disease of the pancreas that can lead to lung injury. Despite extensive research, the mechanisms underlying this complication are ill-defined. In recent years, in vitro co-culture systems have emerged as powerful tools for studying complex interactions between different cell types in disease. In the context of pancreatitis, pancreatic acinar epithelial cells produce and secrete digestive enzymes, and their cellular damage, death, and/or dysfunction is a major contributing factor to the onset of pancreatitis. Here, in this chapter we describe a co-culture system of acinar cells and lung epithelial progenitor/stem cells to model for lung injury associated with pancreatitis.

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Acknowledgements

B.J.J. is supported by a National Health and Medical Research Council of Australia (NHMRC) Senior Research Fellowship (APP1154279). This work was also financially supported in part through the Victorian State Government Operational Infrastructure Support Scheme. We would like to thank Associate Professor Rebecca Lim (Hudson Institute of Medical Research, Melbourne, Australia) and her team for their technical assistance in setting up 3D lung organoids.

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Correspondence to Mohamed I. Saad .

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Saad, M.I., Jenkins, B.J. (2023). An In Vitro Model for Assessing Acute Lung Injury During Pancreatitis Development Using Primary Mouse Cell Co-cultures. In: Jenkins, B.J. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 2691. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3331-1_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3331-1_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3330-4

  • Online ISBN: 978-1-0716-3331-1

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