Abstract
Acute pancreatitis is a serious inflammatory disease of the pancreas that can lead to lung injury. Despite extensive research, the mechanisms underlying this complication are ill-defined. In recent years, in vitro co-culture systems have emerged as powerful tools for studying complex interactions between different cell types in disease. In the context of pancreatitis, pancreatic acinar epithelial cells produce and secrete digestive enzymes, and their cellular damage, death, and/or dysfunction is a major contributing factor to the onset of pancreatitis. Here, in this chapter we describe a co-culture system of acinar cells and lung epithelial progenitor/stem cells to model for lung injury associated with pancreatitis.
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References
Lee PJ, Papachristou GI (2019) New insights into acute pancreatitis. Nat Rev Gastroenterol Hepatol 16:479–496
Weiss FU, Laemmerhirt F, Lerch MM (2019) Etiology and risk factors of acute and chronic pancreatitis. Visc Med 35:73–81
Vidarsdottir H, Möller PH, Vidarsdottir H et al (2013) Acute pancreatitis: a prospective study on incidence, etiology, and outcome. Eur J Gastroenterol Hepatol 25:1068–1075
Habtezion A (2015) Inflammation in acute and chronic pancreatitis. Curr Opin Gastroenterol 31:395–399
Wu BU, Banks PA (2013) Clinical management of patients with acute pancreatitis. Gastroenterol 144:1272–1281
Akbarshahi H, Rosendahl AH, Westergren-Thorsson G et al (2012) Acute lung injury in acute pancreatitis–awaiting the big leap. Respir Med 106:1199–1210
Elder AS, Saccone GT, Dixon D-L (2012) Lung injury in acute pancreatitis: mechanisms underlying augmented secondary injury. Pancreatol 12:49–56
Bhatia M, Zemans RL, Jeyaseelan S (2012) Role of chemokines in the pathogenesis of acute lung injury. Am J Respir Cell Mol Biol 46:566–572
Zhang H, Neuhöfer P, Song L et al (2013) IL-6 trans-signaling promotes pancreatitis-associated lung injury and lethality. J Clin Invest 123:1019–1031
Gout J, Pommier RM, Vincent DF et al (2013) Isolation and culture of mouse primary pancreatic acinar cells. J Vis Exp 78:e50514
Saad MI, Weng T, Lundy J et al (2022) Blockade of the protease ADAM17 ameliorates experimental pancreatitis. Proc Natl Acad Sci USA 119:e2213744119
Leeman KT, Fillmore CM, Kim CF (2014) Lung stem and progenitor cells in tissue homeostasis and disease. Curr Top Dev Biol 107:207–233
Tan JL, Lau SN, Leaw B et al (2018) Amnion epithelial cell-derived exosomes restrict lung injury and enhance endogenous lung repair. Stem Cells Transl Med 7:180–196
Zhu D, Kusuma GD, Schwab R et al (2020) Prematurity negatively affects regenerative properties of human amniotic epithelial cells in the context of lung repair. Clin Sci 134:2665–2679
Kim CFB, Jackson EL, Woolfenden AE et al (2005) Identification of bronchioalveolar stem cells in normal lung and lung cancer. Cell 121:823–835
Acknowledgements
B.J.J. is supported by a National Health and Medical Research Council of Australia (NHMRC) Senior Research Fellowship (APP1154279). This work was also financially supported in part through the Victorian State Government Operational Infrastructure Support Scheme. We would like to thank Associate Professor Rebecca Lim (Hudson Institute of Medical Research, Melbourne, Australia) and her team for their technical assistance in setting up 3D lung organoids.
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Saad, M.I., Jenkins, B.J. (2023). An In Vitro Model for Assessing Acute Lung Injury During Pancreatitis Development Using Primary Mouse Cell Co-cultures. In: Jenkins, B.J. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 2691. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3331-1_6
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DOI: https://doi.org/10.1007/978-1-0716-3331-1_6
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