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Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease

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Inflammation and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2691))

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Abstract

Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.

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References

  1. Bach JF (2002) The effect of infections on susceptibility to autoimmune and allergic diseases. N Engl J Med 347:911–920

    Article  PubMed  Google Scholar 

  2. Dinse GE, Parks CG, Weinberg CR et al (2022) Increasing prevalence of antinuclear antibodies in the United States. Arthritis Rheumatol 74:2032–2041

    Article  CAS  PubMed  Google Scholar 

  3. Mayer-Davis EJ, Lawrence JM, Dabelea D et al (2017) Incidence trends of type 1 and type 2 diabetes among youths, 2002-2012. N Engl J Med 376:1419–1429

    Article  PubMed  PubMed Central  Google Scholar 

  4. Leon BM, Maddox TM (2015) Diabetes and cardiovascular disease: epidemiology, biological mechanisms, treatment recommendations and future research. World J Diabetes 6:1246–1258

    Article  PubMed  PubMed Central  Google Scholar 

  5. Peters MJ, van Halm VP, Voskuyl AE et al (2009) Does rheumatoid arthritis equal diabetes mellitus as an independent risk factor for cardiovascular disease? A prospective study. Arthritis Rheum 61:1571–1579

    Article  PubMed  Google Scholar 

  6. Hansildaar R, Vedder D, Baniaamam M et al (2021) Cardiovascular risk in inflammatory arthritis: rheumatoid arthritis and gout. Lancet Rheumatol 3:e58–e70

    Article  CAS  PubMed  Google Scholar 

  7. Jones GW, Hill DG, Sime K et al (2018) In vivo models for inflammatory arthritis. Methods Mol Biol 1725:101–118

    Article  CAS  PubMed  Google Scholar 

  8. Benson RA, McInnes IB, Garside P et al (2018) Model answers: rational application of murine models in arthritis research. Eur J Immunol 48:32–38

    Article  CAS  PubMed  Google Scholar 

  9. Brand DD, Latham KA, Rosloniec EF (2007) Collagen-induced arthritis. Nat Protoc 2:1269–1275

    Article  CAS  PubMed  Google Scholar 

  10. Reynolds S, Williams AS, Williams H et al (2012) Contractile, but not endothelial, dysfunction in early inflammatory arthritis: a possible role for matrix metalloproteinase-9. Br J Pharmacol 167:505–514

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Davies R, Williams J, Sime K et al (2021) The role of interleukin-6 trans-signalling on cardiovascular dysfunction in inflammatory arthritis. Rheumatology (Oxford) 60:2852–2861

    Article  CAS  PubMed  Google Scholar 

  12. Prati C, Berthelot A, Wendling D et al (2011) Endothelial dysfunction in rat adjuvant-induced arthritis: up-regulation of the vascular arginase pathway. Arthritis Rheum 63:2309–2317

    Article  CAS  PubMed  Google Scholar 

  13. Cai S, Khoo J, Mussa S et al (2005) Endothelial nitric oxide synthase dysfunction in diabetic mice: importance of tetrahydrobiopterin in eNOS dimerisation. Diabetologia 48:1933–1940

    Article  CAS  PubMed  Google Scholar 

  14. Wenceslau CF, McCarthy CG, Earley S et al (2021) Guidelines for the measurement of vascular function and structure in isolated arteries and veins. Am J Physiol Heart Circ Physiol 321:H77–H111

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. du Sert NP, Bamsey I, Bate ST et al (2017) The experimental design assistant. Nat Methods 14:1024–1025

    Article  PubMed  Google Scholar 

  16. Faul F, Erdfelder E, Lang AG et al (2007) G*power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 39:175–191

    Article  PubMed  Google Scholar 

  17. Percie du Sert N, Hurst V, Ahluwalia A et al (2020) The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. BMJ Open Sci 4:e100115

    PubMed  PubMed Central  Google Scholar 

  18. Brackertz D, Mitchell GF, Mackay IR (1977) Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice. Arthritis Rheum 20:841–850

    Article  CAS  PubMed  Google Scholar 

  19. Inglis JJ, Criado G, Medghalchi M et al (2007) Collagen-induced arthritis in C57BL/6 mice is associated with a robust and sustained T-cell response to type II collagen. Arthritis Res Ther 9:R113

    Article  PubMed  PubMed Central  Google Scholar 

  20. Arnal JF, Bayard F (2001) Vasculoprotective effects of oestrogens. Clin Exp Pharmacol Physiol 28:1032–1034

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by funding from Versus Arthritis (reference 22706). SE is supported by a Fight for Sight and Versus Arthritis jointly funded PhD scholarship (reference 5149 / 5150) and ASM by an industry sponsored PhD studentship from Galecto Biotech AB.

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Correspondence to Gareth W. Jones .

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Morrin, A.S., Eastham, S., Williams, A.S., Jones, G.W. (2023). Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease. In: Jenkins, B.J. (eds) Inflammation and Cancer. Methods in Molecular Biology, vol 2691. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3331-1_10

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  • DOI: https://doi.org/10.1007/978-1-0716-3331-1_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3330-4

  • Online ISBN: 978-1-0716-3331-1

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