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Co-immunoprecipitation-Based Identification of Effector–Host Protein Interactions from Pathogen-Infected Plant Tissue

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Protein-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2690))

Abstract

Protein–protein interactions play an essential role in host–pathogen interactions. Phytopathogens secrete a cocktail of effector proteins to suppress plant immunity and reprogram host cell metabolism in their favor. Identification and characterization of effectors and their target protein complexes by co-immunoprecipitation can help to gain a deeper understanding of the functions of individual effectors during pathogenicity and can also provide new insights into the wiring of plant signaling pathways or metabolic complexes. Here we describe a detailed protocol to perform co-immunoprecipitation of effector–target protein complexes from plant extracts with an example of the Ustilago maydis/maize pathosystem for which we also provide a fungal protoplast transformation and maize seedling infection protocols.

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Acknowledgments

We thank Dr. Natalia De Sousa Teixeira E. Silva for providing the U. maydis cells for microscopy and Dr. Aladar Pettkó-Szandtner for helpful comments on the co-IP protocol. Our research is supported by funding from the German Research Foundation (DFG) under Germany’s Excellence Strategy – EXC-2070 – 390732324 (PhenoRob) and DJ 64/5-1 and the Austrian Science Fund (FWF) (I 3033-B22).

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Authors and Affiliations

  1. Department of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany

    Mamoona Khan & Armin Djamei

Authors
  1. Mamoona Khan
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  2. Armin Djamei
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Correspondence to Mamoona Khan .

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Editors and Affiliations

  1. Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA

    Shahid Mukhtar

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Khan, M., Djamei, A. (2023). Co-immunoprecipitation-Based Identification of Effector–Host Protein Interactions from Pathogen-Infected Plant Tissue. In: Mukhtar, S. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 2690. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3327-4_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3327-4_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3326-7

  • Online ISBN: 978-1-0716-3327-4

  • eBook Packages: Springer Protocols

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