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Analyzing Protein–Protein Interactions Using the Split-Ubiquitin System

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Protein-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2690))

Abstract

The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting protein partners. With the introduction of mating-based methods for split-ubiquitin screens, the approach has gained broad popularity because of its exceptionally high transformation efficiency, utility in working with full-length membrane proteins, and positive selection with little interference from spurious interactions. Recent advances now extend these split-ubiquitin methods to the analysis of interactions between otherwise soluble proteins and tripartite protein interactions.

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Acknowledgments

We thank Lingfeng Xia for helping with the figures and for proofreading the text. Funding from the UK Biotechnology and Biological Sciences Research Council and the Royal Society of London is acknowledged.

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Authors and Affiliations

  1. Laboratory of Plant Physiology and Biophysics, School of Molecular Biosciences, University of Glasgow, Glasgow, UK

    Rucha Karnik & Michael R. Blatt

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  1. Rucha Karnik
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  2. Michael R. Blatt
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Corresponding author

Correspondence to Michael R. Blatt .

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Editors and Affiliations

  1. Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA

    Shahid Mukhtar

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Karnik, R., Blatt, M.R. (2023). Analyzing Protein–Protein Interactions Using the Split-Ubiquitin System. In: Mukhtar, S. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 2690. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3327-4_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3327-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3326-7

  • Online ISBN: 978-1-0716-3327-4

  • eBook Packages: Springer Protocols

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