Abstract
Bimolecular fluorescence complementation (BiFC) assay is a method to visualize the protein–protein interaction in living cells. This technique is based on ability of the non-fluorescent fragment of fluorescent protein to form fluorescent complex when they are fused to two interacting proteins. In this chapter, we describe the widely used split yellow fluorescent protein (YFP) system to visualize the protein–protein interaction in plant cells.
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References
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Gnanasekaran, P., Pappu, H.R. (2023). Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein–Protein Interactions in Living Cells. In: Mukhtar, S. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 2690. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3327-4_11
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DOI: https://doi.org/10.1007/978-1-0716-3327-4_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3326-7
Online ISBN: 978-1-0716-3327-4
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