Abstract
Pull-down assay is a technique to analyze direct protein–protein interaction under in vitro condition. Also, this technique is appropriate for investigating the direct interaction between two purified proteins. Glutathione-s-transferase (GST) protein is a widely used affinity tag for affinity purification. In this chapter, we explain the widely used GST pull-down assay to identify the protein–protein interaction between purified proteins.
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Gnanasekaran, P., Pappu, H.R. (2023). Detection of Protein–Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique. In: Mukhtar, S. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 2690. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3327-4_10
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DOI: https://doi.org/10.1007/978-1-0716-3327-4_10
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3326-7
Online ISBN: 978-1-0716-3327-4
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