Abstract
Developing a suitable DNA profile from forensic evidence has long been a lengthy, multi-step laboratory process. Over the last couple of decades, the “process” has exploded into a plethora of numerous options for each of the individual steps, including different manufacturers and commercial kits, as well as options for manual, semi-automated, and automated processing. Despite these options, the heart of the big picture process remains fairly consistent with its early 2000s counterpart and is deeply embedded with a wide variety of precautions to help prevent contamination and ensure integrous results. This includes habitual cleaning, wearing personal protective equipment (PPE), using sterile products and reagents, processing controls, and employing strategic laboratory practices. This chapter serves to briefly introduce new audiences to the forensic DNA process, particularly from a laboratory perspective. Invaluable information regarding routine precautions is included here, and it is highly recommended that this chapter be read first, as much of the information applies to nearly all the chapters of this text.
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References
Federal Bureau of Investigation (2020) Quality Assurance Standards for Forensic DNA Testing Laboratories. Available via the Federal Bureau of Investigation. https://www.fbi.gov/file-repository/quality-assurance-standards-for-forensic-dna-testing-laboratories.pdf/view. Accessed 19 July 2022
Federal Bureau of Investigation (2020) Quality Assurance Standards for Forensic DNA Databasing Laboratories. Available via the Federal Bureau of Investigation. https://www.fbi.gov/file-repository/quality-assurance-standards-for-dna-databasing-laboratories.pdf. Accessed 19 July 2022
Federal Bureau of Investigation (2020) Standards for the Operation of Rapid DNA Booking Systems by Law Enforcement Booking Agencies. Available via the Federal Bureau of Investigation. https://www.fbi.gov/file-repository/standards-for-operation-of-rapid-dna-booking-systems-by-law-enforcement-booking-agencies-eff-090120.pdf. Accessed 19 July 2022
Connon CC, LeFebvre AK, Benjamin RC (2016) Validation of low volume, fast PCR amplification of STR loci for DNA reference samples. J Forensic Legal Investig Sci. https://doi.org/10.24966/FLIS-733X/100008
Connon CC, LeFebvre AK, Benjamin RC (2016) Development of a normalized extraction to further aid in fast, high-throughput processing of forensic DNA reference samples. Forensic Sci Int Genet 25:112–124. https://doi.org/10.1016/j.fsigen.2016.07.019
American Society for Testing and Materials (2017) Standard specification for reagent water. Available via ASTM International. https://www.astm.org/d1193-99e01.html. Accessed 21 July 2022
Burdg J (2015) Infographic: What water type should I use? Available via LabConco. https://www.labconco.com/articles/water-type-difference. Accessed 21 July 2022
Red T (2017) Ultra pure vs feed water, comparing the 4 types of laboratory water. Available via Technology Networks. https://www.technologynetworks.com/immunology/lists/4-types-of-laboratory-water-made-simple-293547. Accessed 21 July 2022
ELGA LabWater (2021) Different types of pure water for the lab: what you need to know. Available via ELGA LabWater. https://www.elgalabwater.com/blog/different-types-pure-water-lab-what-you-need-know. Accessed 21 July 2022
SMACgig Technologies (2022) Methods for making Type1 (Ultrapure) water. Available via SMACgig Technologies. https://www.smacgigworld.com/blog/methods-for-making-ultrapure-type1-water.php. Accessed 21 July 2022
PureTec Industrial Water (2022) What is Deionized Water? Available via PureTec Industrial Water. https://puretecwater.com/deionized-water/what-is-deionized-water#:~:text=Demineralization%20therefore%20requires%20using%20at,is%20always%20first%20in%20line. Accessed 21 July 2022
Grainger (2020) How Does Autoclave Sterilization Work? Available via Grainger. https://www.grainger.com/know-how/equipment-information/kh-how-does-autoclave-sterilization-work. Accessed 21 July 2022
Environmental Protection Agency (1999) Wastewater Technology Fact Sheet: Ozone Disinfection. Available via Environmental Protection Agency. https://www3.epa.gov/npdes/pubs/ozon.pdf. Accessed 21 July 2022
Wikipedia (2022) Purified water. Available via Wikipedia. https://en.wikipedia.org/wiki/Purified_water. Accessed 21 July 2022
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Connon, C.C. (2023). Forensic DNA Analysis: An Overview of the Laboratory Process. In: Cupples Connon, C. (eds) Forensic DNA Analysis. Methods in Molecular Biology, vol 2685. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3295-6_1
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DOI: https://doi.org/10.1007/978-1-0716-3295-6_1
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