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Circulating Tumor Cell Cluster Sorting by Size and Asymmetry

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Microfluidic Systems for Cancer Diagnosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2679))

Abstract

Circulating tumor cell (CTC) clusters are rare yet potent initiators of metastasis and may be useful as clinical biomarkers. Numerous techniques have been developed to isolate individual circulating tumor cells from the blood, but these techniques are often ineffective at capturing CTC clusters and may cause cluster damage or dissociation during processing or recovery. This chapter describes methods for fabricating and operating a two-stage continuous microfluidic chip that isolates and recovers viable CTC clusters from blood or biological fluids using deterministic lateral displacement.

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Acknowledgments

Many thanks to J. Edd, A. Stoddard, K. Wong, F. Fachin, S. Maheswaran, D. Haber, S. Stott, R. Kapur, and M. Toner for their work and contributions to the original manuscript describing the development of this technique.

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Authors and Affiliations

  1. Department of Bioengineering, Imperial College London, London, UK

    Sam H. Au

  2. Cancer Research UK Convergence Science Centre, London, UK

    Sam H. Au

Authors
  1. Sam H. Au
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Correspondence to Sam H. Au .

Editor information

Editors and Affiliations

  1. Institute of Human Biology (IHB), Roche Pharma Research and Early Development, Roche Innovation Center, Basel, Switzerland

    Jose L. Garcia-Cordero

  2. Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA

    Alexander Revzin

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Au, S.H. (2023). Circulating Tumor Cell Cluster Sorting by Size and Asymmetry. In: Garcia-Cordero, J.L., Revzin, A. (eds) Microfluidic Systems for Cancer Diagnosis . Methods in Molecular Biology, vol 2679. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3271-0_2

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  • DOI: https://doi.org/10.1007/978-1-0716-3271-0_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3270-3

  • Online ISBN: 978-1-0716-3271-0

  • eBook Packages: Springer Protocols

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