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Secretion Function Analysis of Ex Vivo Immune Cells in an Integrated Microfluidic Device

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Microfluidic Systems for Cancer Diagnosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2679))

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Abstract

Immune cells play a major role in the development of cancer, from being able to inhibit it by secreting pro-inflammatory mediators, to assist in its development by secreting growth factors, immunosuppressive mediators, and ECM-modifying enzymes. Therefore, the ex vivo analysis of the secretion function of immune cells can be employed as a reliable prognostic biomarker in cancer. However, one limiting factor in current approaches to probe the ex vivo secretion function of cells is their low throughput and the consumption of large quantities of sample. Microfluidics provides a unique advantage, by being able to integrate different components, such as cell culture and biosensors in a monolithic microdevice; it can increase the analytical throughput and leverage it with its intrinsic low sample requirement. Furthermore, the integration of fluid control elements also allows this analysis to be highly automatable, leading to increases in consistency in the results. Here, we describe an approach to analyze the ex vivo secretion function of immune cells using a highly integrated microfluidic device.

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Competing Interests

J.L.G.C is currently employed by F. Hofmann La Roche.

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Authors and Affiliations

  1. Laboratory of Microtechnologies Applied to Biomedicine, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Monterrey, NL, Mexico

    Roberto Rodriguez-Moncayo & Jose L. Garcia-Cordero

  2. Research Laboratory of Electronics, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA

    Roberto Rodriguez-Moncayo

  3. Institute of Human Biology (IHB), Roche Pharma Research and Early Development, Roche Innovation Center, Basel, Switzerland

    Jose L. Garcia-Cordero

Authors
  1. Roberto Rodriguez-Moncayo
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  2. Jose L. Garcia-Cordero
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Corresponding author

Correspondence to Jose L. Garcia-Cordero .

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Editors and Affiliations

  1. Institute of Human Biology (IHB), Roche Pharma Research and Early Development, Roche Innovation Center, Basel, Switzerland

    Jose L. Garcia-Cordero

  2. Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA

    Alexander Revzin

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Rodriguez-Moncayo, R., Garcia-Cordero, J.L. (2023). Secretion Function Analysis of Ex Vivo Immune Cells in an Integrated Microfluidic Device. In: Garcia-Cordero, J.L., Revzin, A. (eds) Microfluidic Systems for Cancer Diagnosis . Methods in Molecular Biology, vol 2679. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3271-0_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3271-0_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3270-3

  • Online ISBN: 978-1-0716-3271-0

  • eBook Packages: Springer Protocols

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