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Methods to Analyze Nutritional and Inter-Organ Control of Drosophila Ovarian Germline Stem Cells

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Germline Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2677))

Abstract

Physiological status, particularly dietary input, has major impacts on the Drosophila melanogaster ovarian germline stem cell lineage. Moreover, several studies have shed light on the role that inter-organ communication plays in coordinating whole-organism responses to changes in physiology. For example, nutrient-sensing signaling pathways function within the fat body to regulate germline stem cells and their progeny in the ovary. Together with its incredible genetic and cell biological toolkits, Drosophila serves as an amenable model organism to use for uncovering molecular mechanisms that underlie physiological control of adult stem cells. In this methods chapter, we describe a general dietary manipulation paradigm, genetic manipulation of adult adipocytes, and whole-mount ovary immunofluorescence to investigate physiological control of germline stem cells.

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Authors and Affiliations

  1. Department of Biological Sciences, University of South Carolina, Columbia, SC, USA

    Chad Simmons, Tancia W. Bradshaw & Alissa R. Armstrong

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  1. Chad Simmons
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  2. Tancia W. Bradshaw
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  3. Alissa R. Armstrong
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Correspondence to Alissa R. Armstrong .

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Editors and Affiliations

  1. Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX, USA

    Michael Buszczak

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Simmons, C., Bradshaw, T.W., Armstrong, A.R. (2023). Methods to Analyze Nutritional and Inter-Organ Control of Drosophila Ovarian Germline Stem Cells. In: Buszczak, M. (eds) Germline Stem Cells. Methods in Molecular Biology, vol 2677. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3259-8_4

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  • DOI: https://doi.org/10.1007/978-1-0716-3259-8_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3258-1

  • Online ISBN: 978-1-0716-3259-8

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