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Diabetic Retinopathy pp 207–217Cite as

Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy

Part of the Methods in Molecular Biology book series (MIMB,volume 2678)

Abstract

This protocol describes a novel approach harnessing the technology of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9-based gene editing for treating retinal angiogenesis. In this system, adeno-associated virus (AAV)-mediated CRISPR/Cas9 was employed to edit the genome of vascular endothelial growth factor receptor (VEGFR)2 in retinal vascular endothelial cells in a mouse model of oxygen-induced retinopathy. The results showed that genome editing of VEGFR2 suppressed pathological retinal angiogenesis. This mouse model mimics a critical aspect of abnormal retinal angiogenesis in patients with neovascular diabetic retinopathy and retinopathy of prematurity, indicating genome editing has high potential for treating angiogenesis-associated retinopathies.

Key words

  • CRISPR/Cas9
  • Vascular endothelial cells
  • Angiogenesis
  • Oxygen-induced retinopathy

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81900893) to WW, the Science and Technology Plan Project of Hunan Province (2019RS2011) (WW), the National Natural Science Foundation of China (82070989) to HL, and Introduction Plan of High-Level Foreign Experts (G2022026027L) to HL. No funding bodies had any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China

    Wenyi Wu

  2. Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, Shenzhen, China

    Hetian Lei

  3. Department of Ophthalmology, Hunan Key Laboratory of Ophthalmology, Changsha, China

    Wenyi Wu

  4. National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China

    Wenyi Wu

  5. Department of Ophthalmology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, China

    Hetian Lei

Authors
  1. Wenyi Wu
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  2. Hetian Lei
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Corresponding author

Correspondence to Hetian Lei .

Editor information

Editors and Affiliations

  1. Centre for Eye Research Australia, University of Melbourne, East Melbourne, VIC, Australia

    Guei-Sheung Liu

  2. Centre for Eye Research Australia, University of Melbourne, East Melbourne, VIC, Australia

    Jiang-Hui Wang

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Cite this protocol

Wu, W., Lei, H. (2023). Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy. In: Liu, GS., Wang, JH. (eds) Diabetic Retinopathy. Methods in Molecular Biology, vol 2678. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3255-0_17

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  • DOI: https://doi.org/10.1007/978-1-0716-3255-0_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3254-3

  • Online ISBN: 978-1-0716-3255-0

  • eBook Packages: Springer Protocols

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