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Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation

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Metabolic Reprogramming

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2675))

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Abstract

Hypoxia and its signalling pathway play a key role in human physiology and a variety of diseases. Alterations in histone methylation coordinate transcriptional responses to hypoxia. Here, we detail a fixed cell immunofluorescence method for quantifying hypoxia-induced changes in histone methylation, exemplified by the measurement of H3K27me3.

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References

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Authors and Affiliations

  1. Department of Biochemistry and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK

    Dilem Shakir, Michael Batie & Sonia Rocha

Authors
  1. Dilem Shakir
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  2. Michael Batie
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  3. Sonia Rocha
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Corresponding author

Correspondence to Sonia Rocha .

Editor information

Editors and Affiliations

  1. Leeds Institute of Medical Research, St. James’s University Hospital, University of Leeds, Leeds, UK

    Salvatore Papa

  2. Center for Genome Engineering and Maintenance, Department of Life Sciences, College of Health, Medicine and Life Sciences, Brunel University London, London, UK

    Concetta Bubici

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Shakir, D., Batie, M., Rocha, S. (2023). Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation. In: Papa, S., Bubici, C. (eds) Metabolic Reprogramming. Methods in Molecular Biology, vol 2675. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3247-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3247-5_19

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3246-8

  • Online ISBN: 978-1-0716-3247-5

  • eBook Packages: Springer Protocols

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