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Isolation of Adipose Tissue Extracellular Vesicles

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Thermogenic Fat

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2662))

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Abstract

Extracellular vesicles (EVs) produced by adipocytes and other adipose tissue (AT) cells are present in the space between cells in the tissue and in circulation. These EVs have been shown to robustly signal between cells in the tissue and in distal organs. AT has unique biophysical properties that require an optimized EV isolation protocol that ensures an uncontaminated EV isolate. This protocol can be used to isolate and characterize the total, heterogeneous population of EVs from the AT.

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References

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Author information

Authors and Affiliations

  1. Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO, USA

    Jessica M. Sabio & Clair Crewe

  2. Division of Endocrinology, Metabolism & Lipid Research, Department of Medicine, Washington University School of Medicine, St Louis, MO, USA

    Clair Crewe

Authors
  1. Jessica M. Sabio
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  2. Clair Crewe
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Corresponding author

Correspondence to Clair Crewe .

Editor information

Editors and Affiliations

  1. School of Medicine, Washington University in St. Louis, Saint Louis, MO, USA

    Irfan J. Lodhi

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sabio, J.M., Crewe, C. (2023). Isolation of Adipose Tissue Extracellular Vesicles. In: Lodhi, I.J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 2662. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3167-6_19

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  • DOI: https://doi.org/10.1007/978-1-0716-3167-6_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3166-9

  • Online ISBN: 978-1-0716-3167-6

  • eBook Packages: Springer Protocols

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