Abstract
Extracellular vesicles (EVs) have emerged as a valuable source for disease biomarkers and an alternative drug delivery system due to their ability to carry cargo and target specific cells. Proper isolation, identification, and analytical strategy are required for evaluating their potential in diagnostics and therapeutics. Here, a method is detailed to isolate plasma EVs and analyze their proteomic profiling, combining EVtrap-based high-recovery EV isolation, phase-transfer surfactant method for protein extraction, and mass spectrometry qualitative and quantitative strategies for EV proteome characterization. The pipeline provides a highly effective EV-based proteome analysis technique that can be applied for EV characterization and evaluation of EV-based diagnosis and therapy.
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Acknowledgments
This project has been funded by NIH grants 3RF1AG064250 to W.A.T. and by Purdue Institute for Cancer Research (PICR).
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Lihon, M.V., Hadisurya, M., Wu, X., Iliuk, A., Tao, W.A. (2023). Isolation and Identification of Plasma Extracellular Vesicles Protein Biomarkers. In: Kasid, U.N., Clarke, R. (eds) Cancer Systems and Integrative Biology. Methods in Molecular Biology, vol 2660. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3163-8_14
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DOI: https://doi.org/10.1007/978-1-0716-3163-8_14
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