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Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy

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The Immune Synapse

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2654))

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Abstract

Reflection interference contrast microscopy (RICM) is an optical microscopy technique ideally suited for imaging adhesion. While RICM (and the closely related interference reflection microscopy (IRM)) has been extensively used qualitatively or semiquantitatively to image cells, including immune cells, it can also be used quantitatively to measure membrane to surface distance, especially for model membranes. Here, we present a protocol for RICM and IRM imaging and the details of semiquantitative and quantitative analysis.

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Author information

Authors and Affiliations

  1. Aix Marseille University, CNRS, CINAM, Turing Centre for Living Systems, Marseille, France

    Ahmed Abdelrahman & Kheya Sengupta

  2. PULS Group, Department of Physics, Centre for Computational Materials and Processes, Friedrich Alexander University Erlangen-Nürnberg, IZNF, Erlangen, Germany

    Ana-Sunčana Smith

  3. Group for Computational Life Sciences, Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb, Croatia

    Ana-Sunčana Smith

Authors
  1. Ahmed Abdelrahman
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  2. Ana-Sunčana Smith
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  3. Kheya Sengupta
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Corresponding author

Correspondence to Ana-Sunčana Smith .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Siena, Siena, Italy

    Cosima T. Baldari

  2. Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK

    Michael L. Dustin

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Abdelrahman, A., Smith, AS., Sengupta, K. (2023). Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy. In: Baldari, C.T., Dustin, M.L. (eds) The Immune Synapse. Methods in Molecular Biology, vol 2654. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3135-5_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3135-5_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3134-8

  • Online ISBN: 978-1-0716-3135-5

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