Skip to main content

Motility Assays of Mycoplasma mobile Under Light Microscopy

  • Protocol
  • First Online:
Bacterial and Archaeal Motility

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2646))

  • 517 Accesses

Abstract

Mycoplasma mobile forms a membrane protrusion at a pole as an organelle. M. mobile cells bind to solid surfaces and glide in the direction of the protrusion. In gliding motility, M. mobile cells catch, pull and release sialylated oligosaccharides on host cells. The observation of Mycoplasma species under light microscopy is useful for the analysis of adhesion ability and the motility mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Razin S, Hayflick L (2010) Highlights of mycoplasma research—an historical perspective. Biologicals 38:183–190. https://doi.org/10.1016/j.biologicals.2009.11.008

    Article  CAS  PubMed  Google Scholar 

  2. Grosjean H, Breton M, Sirand-Pugnet P et al (2014) Predicting the minimal translation apparatus: lessons from the reductive evolution of mollicutes. PLoS Genet 10:e1004363. https://doi.org/10.1371/journal.pgen.1004363

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Miyata M, Robinson RC, Uyeda TQP et al (2020) Tree of motility – a proposed history of motility systems in the tree of life. Genes Cells 25:6–21. https://doi.org/10.1111/gtc.12737

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Barré A, de Daruvar A, Blanchard A (2004) MolliGen, a database dedicated to the comparative genomics of Mollicutes. Nucleic Acids Res 32:D307–D310. https://doi.org/10.1093/nar/gkh114

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Weisburg WG, Tully JG, Rose DL et al (1989) A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol 171:6455–6467. https://doi.org/10.1128/jb.171.12.6455-6467.1989

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Miyata M, Hamaguchi T (2016) Prospects for the gliding mechanism of Mycoplasma mobile. Curr Opin Microbiol 29:15–21. https://doi.org/10.1016/j.mib.2015.08.010

    Article  CAS  PubMed  Google Scholar 

  7. Nakamura S, Minamino T (2019) Flagella-driven motility of bacteria. Biomolecules 9:E279. https://doi.org/10.3390/biom9070279

    Article  CAS  Google Scholar 

  8. Miyata M (2010) Unique centipede mechanism of Mycoplasma gliding. Annu Rev Microbiol 64:519–537. https://doi.org/10.1146/annurev.micro.112408.134116

    Article  CAS  PubMed  Google Scholar 

  9. Nishikawa MS, Nakane D, Toyonaga T et al (2019) Refined mechanism of Mycoplasma mobile gliding based on structure, ATPase activity, and sialic acid binding of machinery. mBio 10:e02846–e02819. https://doi.org/10.1128/mBio.02846-19

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Tulum I, Kimura K, Miyata M (2020) Identification and sequence analyses of the gliding machinery proteins from Mycoplasma mobile. Sci Rep 10:3792. https://doi.org/10.1038/s41598-020-60535-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Adan-Kubo J, Uenoyama A, Arata T, Miyata M (2006) Morphology of isolated Gli349, a leg protein responsible for Mycoplasma mobile gliding via glass binding, revealed by rotary shadowing electron microscopy. J Bacteriol 188:2821–2828. https://doi.org/10.1128/JB.188.8.2821-2828.2006

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Hamaguchi T, Kawakami M, Furukawa H, Miyata M (2019) Identification of novel protein domain for sialyloligosaccharide binding essential to Mycoplasma mobile gliding. FEMS Microbiol Lett 366:fnz016. https://doi.org/10.1093/femsle/fnz016

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Kasai T, Hamaguchi T, Miyata M (2015) Gliding motility of Mycoplasma mobile on uniform oligosaccharides. J Bacteriol 197:2952–2957. https://doi.org/10.1128/JB.00335-15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Kasai T, Nakane D, Ishida H et al (2013) Role of binding in Mycoplasma mobile and Mycoplasma pneumoniae gliding analyzed through inhibition by synthesized sialylated compounds. J Bacteriol 195:429–435. https://doi.org/10.1128/JB.01141-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Lesoil C, Nonaka T, Sekiguchi H et al (2010) Molecular shape and binding force of Mycoplasma mobile’s leg protein Gli349 revealed by an AFM study. Biochem Biophys Res Commun 391:1312–1317. https://doi.org/10.1016/j.bbrc.2009.12.023

    Article  CAS  PubMed  Google Scholar 

  16. Metsugi S, Uenoyama A, Adan-Kubo J et al (2005) Sequence analysis of the gliding protein Gli349 in Mycoplasma mobile. Biophysics (Nagoya-shi) 1:33–43. https://doi.org/10.2142/biophysics.1.33

    Article  CAS  PubMed  Google Scholar 

  17. Morio H, Kasai T, Miyata M (2016) Gliding direction of Mycoplasma mobile. J Bacteriol 198:283–290. https://doi.org/10.1128/JB.00499-15

    Article  CAS  PubMed  Google Scholar 

  18. Nagai R, Miyata M (2006) Gliding motility of Mycoplasma mobile can occur by repeated binding to N-acetylneuraminyllactose (sialyllactose) fixed on solid surfaces. J Bacteriol 188:6469–6475. https://doi.org/10.1128/JB.00754-06

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Taishi Kasai .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Kasai, T., Miyata, M. (2023). Motility Assays of Mycoplasma mobile Under Light Microscopy. In: Minamino, T., Miyata, M., Namba, K. (eds) Bacterial and Archaeal Motility. Methods in Molecular Biology, vol 2646. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3060-0_26

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-3060-0_26

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3059-4

  • Online ISBN: 978-1-0716-3060-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics