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A Method for the In Vitro Cytotoxicity Assessment of Anti-cancer Compounds and Materials Using High Content Screening Analysis

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Cancer Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2645))

Abstract

To date, there is a large bottleneck associated with cancer drug design and development: a lack of appropriate methodologies for screening their potential toxicity. This issue not only causes a high attrition rate for these compounds but also slows down the drug discovery process in general. To overcome this problem, robust, accurate, and reproducible methodologies for assessing anti-cancer compounds are essential. Multiparametric technique and high-throughput analysis, in particular, are favored due to the time- and cost-effective way in which they assess large panels of materials, and due to their large informational output. Following extensive work within our group, we have developed a protocol for assessing the toxicity of anti-cancer compounds using a high-content screening and analysis (HCSA) platform, which is both time-effective and reproducible.

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Acknowledgments

We acknowledge the partial financial support from the European Union under H2020 REFINE (ref 761104), Safe-N-MedTech (ref. 814607), and EUNCL (ref 654190).

The authors would also like to thank several colleagues: Drs C. Maguire and G. Hannon from the Nanomedicine Group and LBCAM at Trinity College Dublin and also Drs M. Roesslin and G. Klinkenberg as members of the EUNCL, REFINE, and Safe-N-Medtech European projects for the technical discussions and insight on aspects related to High Content and Throughput Screening.

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Correspondence to Adriele Prina-Mello .

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Tutty, M.A., Prina-Mello, A. (2023). A Method for the In Vitro Cytotoxicity Assessment of Anti-cancer Compounds and Materials Using High Content Screening Analysis. In: Movia, D., Prina-Mello, A. (eds) Cancer Cell Culture. Methods in Molecular Biology, vol 2645. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3056-3_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3056-3_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3055-6

  • Online ISBN: 978-1-0716-3056-3

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